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elisa-based transam nf-κb p65 transcription factor assay kit  (Active Motif)

 
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    Active Motif elisa-based transam nf-κb p65 transcription factor assay kit
    Effect of astragaloside IV (AS-IV) on angiotensin II (Ang II)-induced increases in the protein levels of phosphorylated IκBα (A) and activities of NF-κB (B) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were preincubated with AS-IV (30 µmol/L) or sodium nitroprusside (SNP, 30 µmol/L) in the presence or absence of N G -monomethyl-L-arginine (L-NMMA, 1 mmol/L), LY294002 (10 µmol/L), or ethylene glycol tetraacetic acid (EGTA, 500 nmol/L) for 1 h and then incubated with Ang II (1 µmol/L) for an additional 30 min. The protein levels of phosphorylated IκBα and activities of NF-κB in HUVECs were measured using a cell-based enzyme-linked immunosorbent assay (ELISA) and a specific <t>TransAM</t> NF-κB <t>p65</t> <t>Transcription</t> Factor Assay Kit, respectively. ⁣ ∗ p < 0.05 compared with the control group; † p < 0.05 compared with HUVECs exposed to Ang II in the presence of AS-IV.
    Elisa Based Transam Nf κb P65 Transcription Factor Assay Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa-based transam nf-κb p65 transcription factor assay kit/product/Active Motif
    Average 90 stars, based on 1605 article reviews
    elisa-based transam nf-κb p65 transcription factor assay kit - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "The Role of Ca 2+ /PI3K/Akt/eNOS/NO Pathway in Astragaloside IV–Induced Inhibition of Endothelial Inflammation Triggered by Angiotensin II"

    Article Title: The Role of Ca 2+ /PI3K/Akt/eNOS/NO Pathway in Astragaloside IV–Induced Inhibition of Endothelial Inflammation Triggered by Angiotensin II

    Journal: Mediators of Inflammation

    doi: 10.1155/2024/3193950

    Effect of astragaloside IV (AS-IV) on angiotensin II (Ang II)-induced increases in the protein levels of phosphorylated IκBα (A) and activities of NF-κB (B) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were preincubated with AS-IV (30 µmol/L) or sodium nitroprusside (SNP, 30 µmol/L) in the presence or absence of N G -monomethyl-L-arginine (L-NMMA, 1 mmol/L), LY294002 (10 µmol/L), or ethylene glycol tetraacetic acid (EGTA, 500 nmol/L) for 1 h and then incubated with Ang II (1 µmol/L) for an additional 30 min. The protein levels of phosphorylated IκBα and activities of NF-κB in HUVECs were measured using a cell-based enzyme-linked immunosorbent assay (ELISA) and a specific TransAM NF-κB p65 Transcription Factor Assay Kit, respectively. ⁣ ∗ p < 0.05 compared with the control group; † p < 0.05 compared with HUVECs exposed to Ang II in the presence of AS-IV.
    Figure Legend Snippet: Effect of astragaloside IV (AS-IV) on angiotensin II (Ang II)-induced increases in the protein levels of phosphorylated IκBα (A) and activities of NF-κB (B) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were preincubated with AS-IV (30 µmol/L) or sodium nitroprusside (SNP, 30 µmol/L) in the presence or absence of N G -monomethyl-L-arginine (L-NMMA, 1 mmol/L), LY294002 (10 µmol/L), or ethylene glycol tetraacetic acid (EGTA, 500 nmol/L) for 1 h and then incubated with Ang II (1 µmol/L) for an additional 30 min. The protein levels of phosphorylated IκBα and activities of NF-κB in HUVECs were measured using a cell-based enzyme-linked immunosorbent assay (ELISA) and a specific TransAM NF-κB p65 Transcription Factor Assay Kit, respectively. ⁣ ∗ p < 0.05 compared with the control group; † p < 0.05 compared with HUVECs exposed to Ang II in the presence of AS-IV.

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay, Transcription Factor Assay, Control



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    Effect of astragaloside IV (AS-IV) on angiotensin II (Ang II)-induced increases in the protein levels of phosphorylated IκBα (A) and activities of NF-κB (B) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were preincubated with AS-IV (30 µmol/L) or sodium nitroprusside (SNP, 30 µmol/L) in the presence or absence of N G -monomethyl-L-arginine (L-NMMA, 1 mmol/L), LY294002 (10 µmol/L), or ethylene glycol tetraacetic acid (EGTA, 500 nmol/L) for 1 h and then incubated with Ang II (1 µmol/L) for an additional 30 min. The protein levels of phosphorylated IκBα and activities of NF-κB in HUVECs were measured using a cell-based enzyme-linked immunosorbent assay (ELISA) and a specific TransAM NF-κB p65 Transcription Factor Assay Kit, respectively. ⁣ ∗ p < 0.05 compared with the control group; † p < 0.05 compared with HUVECs exposed to Ang II in the presence of AS-IV.

    Journal: Mediators of Inflammation

    Article Title: The Role of Ca 2+ /PI3K/Akt/eNOS/NO Pathway in Astragaloside IV–Induced Inhibition of Endothelial Inflammation Triggered by Angiotensin II

    doi: 10.1155/2024/3193950

    Figure Lengend Snippet: Effect of astragaloside IV (AS-IV) on angiotensin II (Ang II)-induced increases in the protein levels of phosphorylated IκBα (A) and activities of NF-κB (B) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were preincubated with AS-IV (30 µmol/L) or sodium nitroprusside (SNP, 30 µmol/L) in the presence or absence of N G -monomethyl-L-arginine (L-NMMA, 1 mmol/L), LY294002 (10 µmol/L), or ethylene glycol tetraacetic acid (EGTA, 500 nmol/L) for 1 h and then incubated with Ang II (1 µmol/L) for an additional 30 min. The protein levels of phosphorylated IκBα and activities of NF-κB in HUVECs were measured using a cell-based enzyme-linked immunosorbent assay (ELISA) and a specific TransAM NF-κB p65 Transcription Factor Assay Kit, respectively. ⁣ ∗ p < 0.05 compared with the control group; † p < 0.05 compared with HUVECs exposed to Ang II in the presence of AS-IV.

    Article Snippet: The NF-κB activity was determined using a nonradioactive, ELISA-based TransAM NF-κB p65 transcription factor assay kit (Active Motif, Carlsbad, CA, USA) as we described previously [ ].

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Transcription Factor Assay, Control